Development of Improved Microarry Gene Expression Indexes using the Probe-level Intensities
نویسندگان
چکیده
Several issues occur to analysis of oligonucleotide microarray data, such as MM as internal control for PM and cross-hybridization. An integrated procedure is proposed to address these problems: (1) log2 scale of PM is used for analysis to avoid bias caused by MM; (2) two-way ANOVA is employed to account for probe effect; and (3) one filter using probability of probe fold change beyond a threshold is used to control for cross-hybridization. An angiogenesis study is used for demonstration.
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تاریخ انتشار 2002